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代高通量测序技术(ChIP-seq的高品质的染色质免疫沉淀DNA模板)
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JoVE Journal
Biology
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Please note that all translations are automatically generated.
Click here for the English version.
代高通量测序技术(ChIP-seq的高品质的染色质免疫沉淀DNA模板)
DOI:
10.3791/50286-v
•
09:52 min
•
April 19, 2013
•
Sandra Deliard
,
Jianhua Zhao
,
Qianghua Xia
,
Struan F.A. Grant
2
1
Division of Human Genetics
,
Children’s Hospital of Philadelphia Research Institute
,
2
Department of Pediatrics, Perelman School of Medicine
,
University of Pennsylvania
Chapters
00:05
Title
00:59
Cross-linking Chromatin
02:27
Sonication
04:14
Pre-clearing Chromatin
05:15
Chromatin Immunoprecipitation
07:10
Reversing Cross-link and Purifying DNA
08:12
Results: Generation of a High Quality ChIP Template
09:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
染色质免疫沉淀和超高通量测序(芯片起)的组合可以识别并映射在一个给定的组织或细胞系的蛋白质-DNA相互作用。概述是如何产生一个高质量的ChIP随后的测序模板,使用的经验,例如转录因子TCF7L2。
Tags
ChIP-seq
Chromatin Immunoprecipitation
High-throughput Sequencing
Transcription Factors
TCF7L2
Type 2 Diabetes
Cancer
HCT116
Protein-DNA Interactions
Genome Sequencing
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