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Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

DOI:
10.3791/61980-v

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10:45 min

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April 03, 2021

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, ,
1Institute of Physiology,Christian-Albrechts-University Kiel

Chapters

  • 00:00Introduction
  • 01:02Preparing the Bench for Slicing
  • 03:57Preparation of Dorsal Hippocampal Slices
  • 07:47Results: The Transversal Slice Shows Enhanced Viability Compared to the Coronal Slice
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons.

Tags

Keywords: Acute SlicesDorsal HippocampusWhole-cell RecordingNeuronal ReconstructionDentate GyrusAdult MiceBrain SlicingElectrophysiologyVideo RecordingsTransversal SlicesProtecting SolutionVibratomeCutting SolutionTranscardial PerfusionIncubation ChamberSlice Storage Chamber

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