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Elektroporeringsbasert genetisk modifisering av primære humane pigmentepitelceller ved bruk av Tornerose Transposon-systemet
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Elektroporeringsbasert genetisk modifisering av primære humane pigmentepitelceller ved bruk av Tornerose Transposon-systemet

Article DOI: 10.3791/61987-v • 07:04 min • February 4th, 2021
February 4th, 2021 •
1, 2,3, 4,5, 4, 6, 7, 1, 2,3
1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute

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Vi har utviklet en protokoll for å transfektere primære humane pigmentepitelceller ved elektroporering med genet som koder for pigmentepitelavledet faktor (PEDF) ved hjelp av Tornerose (SB) transposonsystemet. Vellykket transfeksjon ble demonstrert ved kvantitativ polymerasekjedereaksjon (qPCR), immunoblotting og enzymbundet immunosorbentanalyse (ELISA).

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Medisin utgave 168 aldersrelatert makuladegenerasjon AMD kapillær elektroporeringssystem ikke-viral transfeksjon pigmentepitel-avledet faktor PEDF primære pigmentepitelceller Tornerose transposonsystem SB
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