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Journal / Genetics / Direkt omprogrammering av mänskliga fibroblaster…
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JoVE Journal Genetics
Direkt omprogrammering av mänskliga fibroblaster till myoblaster för att undersöka terapier för neuromuskulära störningar
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Direkt omprogrammering av mänskliga fibroblaster till myoblaster för att undersöka terapier för neuromuskulära störningar

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Detta protokoll beskriver omvandlingen av huden fibroblaster till myoblaster och deras differentiering till myotubes. Cellinjerna kommer från patienter med neuromuskulära sjukdomar och kan användas för att undersöka patologiska mekanismer och testa terapeutiska strategier.

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Genetik Utgåva 170 Omprogrammering hTERT MyoD fibroblast myoblast neuromuskulär störning terapitestning
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