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1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University
Chapters
- 00:00Introduction
- 00:36Overview
- 01:23Initiation of Demal Fibroblasts Culture
- 02:46Establishment of FM Cell Lines Lentvirus-hTERT Transduction
- 03:57Lentivirus-MyoD Transduction
- 04:54Fibroblasts Transdifferentiation
- 06:11Results
- 09:16Conclusion
Detta protokoll beskriver omvandlingen av huden fibroblaster till myoblaster och deras differentiering till myotubes. Cellinjerna kommer från patienter med neuromuskulära sjukdomar och kan användas för att undersöka patologiska mekanismer och testa terapeutiska strategier.
