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JoVE Journal Biochemistry
Udnyttelse af grafix til påvisning af forbigående interactorer af saccharomyces cerevisiae splejsning splejsstoffer
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Udnyttelse af grafix til påvisning af forbigående interactorer af saccharomyces cerevisiae splejsning splejsstoffer

Article DOI: 10.3791/61994-v • 05:44 min • November 9th, 2020
November 9th, 2020 •
1, 1, 1, 1, 1
1Department of Biochemistry, Institute of Chemistry, University of Sao Paulo

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Her beskriver vi udnyttelsen af Grafix (Gradient Fiksering), en glycerol gradient centrifugering i nærværelse af en crosslinker, for at identificere interaktioner mellem splejsning faktorer, der binder forbigående til splejsning kompleks.

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Biokemi Udgave 165 gærsplejsiske underkomplekser Grafix adskillelse af RNP-komplekser splejsning Saccharomyces cerevisiae glycerol gradient centrifugering
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