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Journal
/
Biochemistry
/
格拉菲克斯用于检测
糖精
的瞬态相互作用器
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Utilization of Grafix for the Detection of Transient Interactors of
Saccharomyces cerevisiae
Spliceosome Subcomplexes
Please note that all translations are automatically generated.
Click here for the English version.
格拉菲克斯用于检测
糖精
的瞬态相互作用器
DOI:
10.3791/61994-v
•
05:44 min
•
November 09, 2020
•
Felipe A. Carvalho
,
Moises R. A. Barros
,
Thierry P. F. Girotto
,
M. Griselda Perona
,
Carla C. Oliveira
1
Department of Biochemistry, Institute of Chemistry
,
University of Sao Paulo
Chapters
00:04
Introduction
00:45
Yeast Total Extract Preparation
02:07
Glycerol Gradient Preparation
02:58
Extract Separation and Collection
03:44
Results: Representative Transient
Saccharomyces Cerevisiae
Spliceosome Subcomplex Interactor Detection
05:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了Grafix(梯度固定)的利用,这是一种在交行器面前的甘油梯度离心,用于识别暂时与拼接体复合物结合的拼接因子之间的相互作用。
Tags
Grafix
Transient Interactors
Saccharomyces Cerevisiae
Spliceosome Subcomplexes
Gradient Fixation Method
Glycerol Gradient Centrifugation
Cross-linker
Fixation Reagents
Yeast Total Extract Preparation
Tap Tag
Glycerol Gradient
Glutaraldehyde
Gradient Master Device
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