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Isolation og differentiering af primære hvide og brune præadipocytter fra nyfødte mus
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Isolation og differentiering af primære hvide og brune præadipocytter fra nyfødte mus

Article DOI: 10.3791/62005-v • 09:00 min • January 25th, 2021
January 25th, 2021 •
1,2, 1, 1
1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA, 2Department of Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

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Denne rapport beskriver en protokol for samtidig isolering af primære brune og hvide præadipocytter fra nyfødte mus. Isolerede celler kan dyrkes i kultur og induceres til at differentiere sig til fuldt modne hvide og brune adipocytter. Metoden muliggør genetisk, molekylær og funktionel karakterisering af primære fedtceller i kulturen.

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Denne måned i JoVE White adipocytes brune adipocytter mus primær kultur adipogenesis fedme diabetes metabolisk sygdom
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