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Journal / Biology / Primer Beyaz ve Kahverengi Preadipositlerin…
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JoVE Journal Biology
Primer Beyaz ve Kahverengi Preadipositlerin Yenidoğan Farelerden İzolasyonu ve Farklılaşması
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Primer Beyaz ve Kahverengi Preadipositlerin Yenidoğan Farelerden İzolasyonu ve Farklılaşması

Article DOI: 10.3791/62005-v • 09:00 min • January 25th, 2021
January 25th, 2021 •
1,2, 1, 1
1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA, 2Department of Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

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Bu rapor, birincil kahverengi ve beyaz preadipositlerin yenidoğan farelerden eşzamanlı izolasyonu için bir protokol açıklanmaktadır. İzole hücreler kültürde yetiştirilebilir ve tamamen olgun beyaz ve kahverengi adipozitlere farklılaşmaya teşvik edilebilir. Yöntem, kültürdeki primer yağ hücrelerinin genetik, moleküler ve fonksiyonel olarak karakterizeini sağlar.

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JoVE'de Bu Ay Sayı 167 Beyaz adipozitler kahverengi adipositler fare birincil kültür adipogenez obezite diyabet metabolik hastalık
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