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初白和棕色预生细胞与新生小鼠的隔离和分化
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Biology
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JoVE Journal
Biology
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
Please note that all translations are automatically generated.
Click here for the English version.
初白和棕色预生细胞与新生小鼠的隔离和分化
DOI:
10.3791/62005-v
•
09:00 min
•
January 25, 2021
•
Andrea Galmozzi
2
,
Bernard P. Kok
,
Enrique Saez
1
Department of Molecular Medicine
,
The Scripps Research Institute, La Jolla, CA, USA
,
2
Department of Medicine
,
University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA
Chapters
00:04
Introduction
01:32
Adipose Depot Collection and Digestion
03:28
Plating Preadipocytes
04:56
Preadipocyte Culture Expansion
06:17
White and Brown Preadipocyte Differentiation
06:55
Results: Representative Adipocyte Progenitor Collection and Differentiation
08:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本报告描述了一个同时将原生棕色和白色预生细胞与新生小鼠隔离的协议。孤立的细胞可以在培养中生长,并诱导分化成完全成熟的白色和棕色脂肪细胞。该方法使培养中原脂肪细胞的遗传、分子和功能特征化。
Tags
Preadipocytes
Adipocyte Isolation
Adipocyte Differentiation
Newborn Mice
Obesity
Diabetes
Cell Culture Model
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