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JoVE Journal Cancer Research
Kjemisk konjugering av et renset DEC-205-rettet antistoff med protein i full lengde for målretting av musedrittiske celler In Vitro og In Vivo
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Kjemisk konjugering av et renset DEC-205-rettet antistoff med protein i full lengde for målretting av musedrittiske celler In Vitro og In Vivo

Article DOI: 10.3791/62018-v • 10:35 min • February 5th, 2021
February 5th, 2021 •
1,2, 1,2,3, 1, 1,2, 4, 4, *1,2,5, *1,2
1Immune Regulation Group, Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research, Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg
* These authors contributed equally

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Vi beskriver en protokoll for kjemisk konjugering av modellen antigen ovalbumin til en endokytosereseptor-spesifikt antistoff for in vivo dendritisk celle målretting. Protokollen inkluderer rensing av antistoffet, kjemisk konjugering av antigenet, samt rensing av konjugaten og verifisering av effektiv konjugering.

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Kreftforskning Utgave 168 dendrittisk cellemålretting dendrittiske celler DEC-205 antigentilførsel antigenkonjugering kjemisk konjugering antistoffmediert endokytose vaksinasjon antitumorimmunitet
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