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Journal / Cancer Research / Vitro ve In Vivo'da Fare Dendritik Hücrelerini…
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Cancer Research

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JoVE Journal Cancer Research
Vitro ve In Vivo'da Fare Dendritik Hücrelerini Hedeflemek için Tam Uzunlukta Proteinli Saflaştırılmış DEC-205 Yönlendirilmiş Antikorun Kimyasal Konjugasyonu
Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
Journal (Cancer Research)
Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
Journal (Cancer Research)
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
 
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Vitro ve In Vivo'da Fare Dendritik Hücrelerini Hedeflemek için Tam Uzunlukta Proteinli Saflaştırılmış DEC-205 Yönlendirilmiş Antikorun Kimyasal Konjugasyonu

Article DOI: 10.3791/62018-v • 10:35 min • February 5th, 2021
February 5th, 2021 •
1,2, 1,2,3, 1, 1,2, 4, 4, *1,2,5, *1,2
1Immune Regulation Group, Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research, Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg
* These authors contributed equally

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Model antijen ovalbumin'in in vivo dendritik hücre hedeflemesi için endositoz reseptörüne özgü antikora kimyasal konjugasyonu için bir protokol açıklıyoruz. Protokol, antikorun saflaştırılmasını, antijenin kimyasal konjugasyonunu, ayrıca konjugenin saflaştırılmasını ve verimli konjugasyonun doğrulanmasını içerir.

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Kanser Araştırması Sayı 168 dendritik hücre hedefleme dendritik hücreler DEC-205 antijen doğumu antijen konjugasyonu kimyasal konjugasyon antikor aracılı endositoz aşılama antitümör bağışıklık
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