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Semikvantitativ bestämning av dopaminerg neurontäthet i substantia nigra av gnagaremodeller med hjälp av automatiserad bildanalys

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

Semikvantitativ bestämning av dopaminerg neurontäthet i substantia nigra av gnagaremodeller med hjälp av automatiserad bildanalys

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4,440 Views

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06:09 min

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February 02, 2021

DOI:

10.3791/62062-v

DOI:

10.3791/62062-v

•

06:09 min

February 02, 2021

•

4427 Views

Darren M. O’Hara^1,2, Minesh Kapadia^1,2, Susan Ping^1,2, Suneil K. Kalia^1,2,3, Lorraine V. Kalia^1,2,4,5,6

¹Krembil Research Institute, Toronto Western Hospital,University Health Network ²Department of Laboratory Medicine and Pathobiology,University of Toronto ³Department of Surgery, Division of Neurosurgery,University of Toronto ⁴Department of Medicine, Division of Neurology,University of Toronto ⁵Department of Medicine, Division of Neurology, Edmond J. Safra Program in Parkinson’s Disease and the Morton and Gloria Shulman Movement Disorders Clinic, Toronto Western Hospital,University Health Network ⁶Tanz Centre for Research in Neurodegenerative Diseases,University of Toronto

Summary

Här presenterar vi en automatiserad metod för semikvantitativ bestämning av dopaminerga neuronnummer i råtta substantia nigra pars compacta.

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