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1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Chapters
- 00:05Introduction
- 00:29Design of Gene-Specific Primers and Preparation of sgRNA and Embryos
- 02:18Microinjection of Embryos
- 03:47Post-Injection Insect Rearing
- 04:41Knock-Out Mutation Detection
- 05:27Results: Selection and Synthesis of Target sgRNAs and Detection of Mutants by PCR and Gel Electrophoresis
- 06:08Conclusion
Præsenteret her er en protokol over Helicoverpa armigera (Hübner) embryomikroinjektion og knockout mutant identifikation skabt af CRISPR / Cas9 genomredigering. Mutante insekter muliggør yderligere forskning i genfunktion og interaktion mellem forskellige gener in vivo.
