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Microiniezione embrionale e identificazione di mutanti knockout di Helicoverpa Armigera (Hübner) modificata dal genoma CRISPR/Cas9
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Microiniezione embrionale e identificazione di mutanti knockout di Helicoverpa Armigera (Hübner) modificata dal genoma CRISPR/Cas9

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Qui è presentato un protocollo di microiniezione embrionale Helicoverpa armigera (Hübner) e identificazione di mutanti knockout creati dall'editing del genoma CRISPR / Cas9. Gli insetti mutanti consentono ulteriori ricerche sulla funzione genica e sull'interazione tra diversi geni in vivo.

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Comportamento Problema 173 Helicoverpa armigera microiniezione embrionale knockout genici CRISPR Cas9
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