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开源微型氟度计，以监测资源有限设置中的实时异热核酸放大反应
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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开源微型氟度计，以监测资源有限设置中的实时异热核酸放大反应

DOI:
10.3791/62148-v

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09:36 min

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February 03, 2021

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, , , , , , ,
1Department of Bioengineering,Rice University

Chapters

  • 00:04Introduction
  • 00:44Optical Housing Assembly
  • 03:39Electronics and Touchscreen Assembly
  • 06:46Real-Time Fluorescence Data Recording
  • 08:08Results: Representative Miniature Fluorimeter Measurements
  • 09:07Conclusion

Summary

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提供详细的说明，以建立一个开源的模块化氟度计，与许多低成本加热器兼容，以执行实时，定量等热核酸放大。

Tags

Keyword Extraction:Open-sourceMiniatureFluorimeterIsothermalNucleic Acid AmplificationResource-limited SettingsMolecular DiagnosticsIsothermal AmplificationOptics EnclosureTest BoardFocal Length LensLong Pass FilterEmission FilterDichroic MirrorBeam SplitterExcitation FilterLED

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