JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
具有可移动XY阶段的平台孵化器:实现蛋白质细胞内快速光化学氧化的新平台
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
Please note that all translations are automatically generated.
Click here for the English version.
具有可移动XY阶段的平台孵化器:实现蛋白质细胞内快速光化学氧化的新平台
DOI:
10.3791/62153-v
•
05:50 min
•
May 17, 2021
•
Danté Johnson
,
Benjamin Punshon-Smith
,
Jessica A. Espino
,
Anne Gershenson
,
Lisa M. Jones
1
Department of Pharmaceutical Sciences
,
University of Maryland Baltimore
,
2
Technology Research Center
,
University of Maryland Baltimore County
,
3
Department of Biochemistry and Molecular Biology
,
University of Massachusetts
Chapters
00:04
Introduction
00:45
Setting Up the Platform Incubator for IC-FPOP
02:08
IC-FPOP in the Platform Incubator
03:41
Results: Analysis of Cell Transfection Efficiency, Proteins Modified in Single Cell Flow-System and PIXY, and Localization of IC-FPOP Modifications
05:22
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
一种新的静态平台用于利用一种称为蛋白质细胞内快速光化学氧化(IC-FPOP)的蛋白质足迹技术,对原生细胞环境中的蛋白质结构和相互作用部位进行特征。
Tags
Platform Incubator
Movable XY Stage
In-cell FPOP
Protein-ligand Interactions
Protein-protein Interactions
Conformational Changes
Automated Six-well Plate
Cell Culture
Hydrogen Peroxide
Quench Buffer
Peristaltic Pumps
Laser
Cell Scraper
Centrifugation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
制造的UV-Vis和拉曼光谱免疫平台
用 [NiFe] 氢对 H
2
氧化的研究表明, 蛋白质膜红外电化学
基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1
大鼠甲基 Seq 平台识别与应力接触相关的表观变化
重组融合蛋白在荧光蛋白酶检测平台中的应用及其凝胶内再生
基于原生自荧光的sFLIM测量植物部分荧光探针和木质素之间的相互作用
用细胞内快速光化学氧化蛋白质对细胞内蛋白质进行特征化
氢-铀交换质谱仪(HDX-MS)平台,用于研究肽生物合成酶
细胞性谷胱甘肽S-转移酶潜在抑制剂的分光光度筛选
成熟水稻的横向切片(
米
苜蓿L.)使用移液器吸头作为固定支架的扫描电子显微镜成像的内核
Read Article