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JoVE Journal Cancer Research
Samkultur av Glioblastoma stamliknande celler på mönstrade nervceller för att studera migration och cellulära interaktioner
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Samkultur av Glioblastoma stamliknande celler på mönstrade nervceller för att studera migration och cellulära interaktioner

Article DOI: 10.3791/62213-v • 10:08 min • February 24th, 2021
February 24th, 2021 •
1, 2,3, 4, 1, *2, *4
1University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, 2Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, 3Alvéole, Bordeaux, France, 4University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France
* These authors contributed equally

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Här presenterar vi en lättanvänd co-culture analys för att analysera glioblastom (GBM) migration på mönstrade nervceller. Vi utvecklade ett makro i FiJi programvara för enkel kvantifiering av GBM cell migrering på nervceller, och observerade att nervceller ändra GBM cell invasiv kapacitet.

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Cancerforskning Utgåva 168 glioblastom patientledd cell sfäroid invasion nervceller avancerade in vitro-modeller
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