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Journal / Immunology and Infection / Purificação e Expansão de Células T Assassinos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Purificação e Expansão de Células T Assassinos Naturais Invariantes para Estudos In vitro e In vivo
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Purificação e Expansão de Células T Assassinos Naturais Invariantes para Estudos In vitro e In vivo

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Descrevemos um protocolo rápido e robusto para enriquecer células T (iNKT) invariantes do assassino natural do baço do rato e expandi-las in vitro para números adequados para estudos in vitro e in vivo.

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Imunologia e Infecção Problema 168 células iNKT expansão mouse Vα14 CD1d contas anti-CD3/CD28 ativação linfócitos
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