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JoVE Journal Behavior
Sammenlignende analyse af eksperimentelle metoder til kvantificering af dyrs aktivitet i Caenorhabditis elegans Modeller af Mitokondriesygdom
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Sammenlignende analyse af eksperimentelle metoder til kvantificering af dyrs aktivitet i Caenorhabditis elegans Modeller af Mitokondriesygdom

Article DOI: 10.3791/62244-v • 05:51 min • April 4th, 2021
April 4th, 2021 •
*1, *1, 1, 1,2, 1,2
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania Perelman School of Medicine
* These authors contributed equally

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Denne undersøgelse præsenterer protokoller for to semi-automatiserede lokomotorisk aktivitet analyse tilgange i C. elegans kompleks I sygdom gas-1(FC21) orme, nemlig ZebraLab (en medium-throughput assay) og WormScan (en high-throughput assay) og give sammenlignende analyse blandt en bred vifte af forskningsmetoder til at kvantificere nematode adfærd og integreret neuromuskulær funktion.

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Adfærd Problem 170 orme C. elegans locomotorisk aktivitet svælg pumpning chemotaxis prygl ZebraLab WormScan high-throughput screening kapacitet gas-1(fc21)
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