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Análise comparativa de métodos experimentais para quantificar a atividade animal em Caenorhabditis elegans Modelos de Doença Mitocondrial
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Análise comparativa de métodos experimentais para quantificar a atividade animal em Caenorhabditis elegans Modelos de Doença Mitocondrial

Article DOI: 10.3791/62244-v • 05:51 min • April 4th, 2021
April 4th, 2021 •
*1, *1, 1, 1,2, 1,2
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania Perelman School of Medicine
* These authors contributed equally

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Este estudo apresenta protocolos para duas abordagens semi-automatizadas de análise de atividade locomotor em C. elegans complexo I disease gas-1(fc21) worms, ou seja, ZebraLab (um ensaio de rendimento médio) e WormScan (um ensaio de alto rendimento) e fornecem análise comparativa entre uma ampla gama de métodos de pesquisa para quantificar o comportamento nematoides e função neuromuscular integrada.

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Comportamento Problema 170 worms C. elegans,atividade locomotor bombeamento faríneal quimotáxi thrashing ZebraLab WormScan capacidade de triagem de alto rendimento gás-1(fc21)
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