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Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
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Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Article DOI: 10.3791/62271-v • 08:59 min • February 25th, 2021
February 25th, 2021 •
1, 2, 1, 1, 1
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software

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The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.

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Cryo-electron Microscopic Grid Preparation Time-resolved Studies Spotiton Robotic System Protein Interaction Electron Microscope Grid Transient Protein Confirmations Sub-second Biological Systems Biochemical Systems Membrane Channel Activation DNA Synthesis RNA Synthesis Drug-protein Interaction Antibody-protein Interaction Nitrogen Supply Tank De-gassed Ultrapure Water Spotiton Software Three Axis Robots Rotating Dispenser Head Assembly Dispenser Tips Safe Position Aspirate Tab
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