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Journal / Biology / Kryo-elektron mikroskopisk gitter forberedelse…
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Biology

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JoVE Journal Biology
Kryo-elektron mikroskopisk gitter forberedelse til tid-løste undersøgelser ved hjælp af en ny robot system, Spotiton
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Kryo-elektron mikroskopisk gitter forberedelse til tid-løste undersøgelser ved hjælp af en ny robot system, Spotiton

Article DOI: 10.3791/62271-v • 08:59 min • February 25th, 2021
February 25th, 2021 •
1, 2, 1, 1, 1
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software

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Protokollen præsenteres her beskriver brugen af Spotiton, en ny robot system, til at levere to prøver af interesse på en selvtransporterende, nanowire gitter, der blandes for mindst 90 ms før vitrifikation i flydende cryogen.

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Biologi Tid-løst kortvarig molekylær stater cryo-EM vitrification piezo dispensering nanowire gitre robot bevægelse
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