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Journal / Chemistry / 使用高细胞密度生物反应器通过实时定量细胞内 NMR…
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Chemistry

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使用高细胞密度生物反应器通过实时定量细胞内 NMR 监测人细胞中蛋白质-配体相互作用
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使用高细胞密度生物反应器通过实时定量细胞内 NMR 监测人细胞中蛋白质-配体相互作用

Article DOI: 10.3791/62323-v • 10:25 min • March 9th, 2021
March 9th, 2021 •
1,2, 1,3
1Magnetic Resonance Center − CERM, University of Florence, 2Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine − CIRMMP, 3Neurofarba Department, University of Florence

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该协议描述了NMR生物反应器的设置，以保持封装的人类细胞存活长达72小时，然后进行时间分辨的细胞内NMR数据采集和分析。该方法用于实时监测细胞内蛋白质 - 配体相互作用。

Tags

化学，第169期，细胞内核磁共振，实时，生物反应器，多变量曲线分辨率，配体结合，碳酸酐酶II，乙酰唑胺，甲唑胺，蛋白质氧化，超氧化物歧化酶1，ebselen
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