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不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）

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Chemistry

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JoVE Journal Chemistry

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

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不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）

DOI:

10.3791/50433-v

•

11:00 min

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May 20, 2013

•

Nathaniel W. Snyder, Maya Khezam, Clementina A. Mesaros, Andrew Worth, Ian A. Blair

¹Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Chapters

00:05Title
01:20Sample Extraction from Cells
02:34Re-suspension and Filtration of Samples for UPLC
03:49UPLC and Mass Spectrometer Setup
06:37Differential Analysis
08:47Results: Analysis of Metabolites Using UPLC-HRMS
10:42Conclusion

Summary

Automatic Translation

不相关的代谢组学研究提供了一个假说，产生一种代谢轮廓的快照。此协议将展示从细胞，血清或组织中的代谢产物的提取和分析。代谢物甲范围进行调查，采用液 - 液固相萃取，微流超高效液相色谱/高分辨率质谱（UPLC-HRMS）耦合到差分分析软件。

Tags

Keywords: Untargeted Metabolomics UPLC-HRMS Liquid-liquid Extraction Polar And Non-polar Metabolites Microflow UPLC Positive And Negative Ionization Label-free Differential Analysis Metabolic Pathway Screening Biomarker Discovery Drug Development

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