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使用高分辨率X射线吸收光谱在低温下制备生物样品以进行形态分析
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
DOI:

06:00 min

May 27, 2022

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Chapters

  • 00:05Introduction
  • 00:51Human PC-3 and OVCAR-3 Cancer Cell Pellet Preparation
  • 02:58High Resolution X-Ray Absorption Spectroscopy (HERFD-XAS) Measuring Procedure
  • 03:40Results: Selenium (Se) and Iron (Fe) K-Edge Typical HERFD-XANES Analyses
  • 04:56Conclusion

Summary

Automatic Translation

该协议提出了为基于同步加速器的X射线吸收光谱实验制备生物冷冻样品的详细程序。我们通过癌症和浮游植物细胞方案的示例描述了优化样品制备和冷冻保存所需的所有步骤。该方法提供了样品冷冻制备的通用标准。

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