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JoVE Journal Bioengineering
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DNA-spanningsondes om de voorbijgaande piconewtonreceptorkrachten door immuuncellen in kaart te brengen

Article DOI: 10.3791/62348-v • 06:53 min • March 20th, 2021
March 20th, 2021 •
1, 2, 1, 1, 2, 1,2
1Department of Chemistry, Emory University, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

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Dit artikel beschrijft een gedetailleerd protocol voor het gebruik van DNA-gebaseerde spanningsondes om de receptorkrachten in beeld te brengen die door immuuncellen worden toegepast. Deze aanpak kan receptorkrachten >4,7pN in realtime in kaart brengen en krachten in de loop van de tijd integreren.

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Deze maand in JoVE DNA tension probes mechanobiology receptor force piconewton forces immune cells mapping
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