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Bioengineering
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DNA张力探针用于绘制免疫细胞的瞬时皮牛顿受体力
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
Please note that all translations are automatically generated.
Click here for the English version.
DNA张力探针用于绘制免疫细胞的瞬时皮牛顿受体力
DOI:
10.3791/62348-v
•
06:53 min
•
March 20, 2021
•
Rong Ma
,
Anna V. Kellner
,
Yuesong Hu
,
Brendan R. Deal
,
Aaron T. Blanchard
,
Khalid Salaita
2
1
Department of Chemistry
,
Emory University
,
2
Wallace H. Coulter Department of Biomedical Engineering
,
Georgia Institute of Technology and Emory University
Chapters
00:04
Introduction
00:41
Surface Preparation and Activation of the Coverslip
04:37
Imaging Cell Receptor Forces
05:21
Results: Estimation of Quality and Functionalization of the DNA Probe Surface
06:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了使用基于DNA的张力探针对免疫细胞施加的受体力进行成像的详细方案。这种方法可以实时映射受体力>4.7pN,并且可以随着时间的推移积分力。
Tags
DNA Tension Probes
Mechanotransduction
Mechanobiology
Fluorescence Microscopy
Force Transmission
Immortalized Cells
Primary Cells
Polytetrafluoroethylene
Piranha Solution
Aminopropyltriethoxysilane
Amine
Lipoic Acid PEG NHS
MPEG NHS
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