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Biochemistry

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JoVE Journal Biochemistry
Preparazione di lamelle da campioni biologici vitreali utilizzando un microscopio elettronico a scansione a doppio raggio per la tomografia crioelettronica
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Preparazione di lamelle da campioni biologici vitreali utilizzando un microscopio elettronico a scansione a doppio raggio per la tomografia crioelettronica

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

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Utilizzo della fresatura a fascio ionico focalizzato per produrre lamelle vetrose su griglia da campioni biologici congelati a immersione per la tomografia crioelettronica.

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Retrazione Numero 174 FIB-milling cryoFIB-SEM lamelle tomografia cryoET cryoEM Plasmodium falciparum
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