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Journal / Biochemistry / Forberedelse af lameller fra glasagtige…
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Biochemistry

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JoVE Journal Biochemistry
Forberedelse af lameller fra glasagtige biologiske prøver ved hjælp af et dobbeltstrålescanningselektronmikroskop til kryo-elektrontomografi
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Forberedelse af lameller fra glasagtige biologiske prøver ved hjælp af et dobbeltstrålescanningselektronmikroskop til kryo-elektrontomografi

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

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Brug af fokuseret ionstrålefræsning til fremstilling af glasagtige lameller på gitteret fra dybfrosne biologiske prøver til kryo-elektrontomografi.

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Tilbagetrækning udgave 174 FIB-fræsning cryoFIB-SEM lameller tomografi cryoET cryoEM Plasmodium falciparum
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