Journal
/
/
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
DOI:

08:09 min

April 19, 2021

, ,

Chapters

  • 00:04Introduction
  • 01:00Initial Nuclear Magnetic Resonance (NMR) Experiment Setup
  • 02:00Routine NMR Experiment Setup
  • 05:53Results: C-Terminal Domain of Bacterial Enzyme I (EIC) s-ms Dynamics
  • 07:10Conclusion

Summary

Automatic Translation

Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of 15N relaxation dispersion profiles by solution NMR spectroscopy is provided.

Related Videos

Read Article