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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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Biochemistry
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JoVE Journal Biochemistry
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

DOI:
10.3791/62395-v

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08:09 min

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April 19, 2021

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, ,
1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Chapters

  • 00:04Introduction
  • 01:00Initial Nuclear Magnetic Resonance (NMR) Experiment Setup
  • 02:00Routine NMR Experiment Setup
  • 05:53Results: C-Terminal Domain of Bacterial Enzyme I (EIC) s-ms Dynamics
  • 07:10Conclusion

Summary

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Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of 15N relaxation dispersion profiles by solution NMR spectroscopy is provided.

Tags

15N CPMGProtein Conformational DynamicsNMR SpectroscopyBiomolecular StructureHSQCPulse ProgramAcquisition ParametersWater SuppressionRelaxation Dispersion

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