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Journal / Biochemistry / Single Particle Cryo-Electron Mikroskopi: Från…
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Biochemistry

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JoVE Journal Biochemistry
Single Particle Cryo-Electron Mikroskopi
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Journal (Biochemistry)
Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids (Video) | JoVE
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Journal (Biochemistry)
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Single Particle Cryo-Electron Mikroskopi: Från prov till struktur

Article DOI: 10.3791/62415-v • 11:52 min • May 29th, 2021
May 29th, 2021 •
1, 1, 2, 2, 2, 2, 1, 1
1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences, University of Leeds, 2Diamond Light Source, Harwell Science and Innovation Campus

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Strukturbestämning av makromolekylära komplex med cryoEM har blivit rutin för vissa klasser av proteiner och komplex. Här sammanfattas denna pipeline (provberedning, screening, datainsamling och bearbetning) och läsarna riktas mot ytterligare detaljerade resurser och variabler som kan ändras vid mer utmanande exemplar.

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Biokemi nummer 171 CryoEM enstaka partikel struktur elektronmikroskopi
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