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Biology
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探索病毒感染时的
m6A
和
m5C
表继转录组:HIV示例
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Exploring m
6
A and m
5
C Epitranscriptomes upon Viral Infection: an Example with HIV
Please note that all translations are automatically generated.
Click here for the English version.
探索病毒感染时的
m6A
和
m5C
表继转录组:HIV示例
DOI:
10.3791/62426-v
•
14:40 min
•
March 05, 2022
•
Sara Cristinelli
,
Paolo Angelino
,
Angela Ciuffi
1
Institute of Microbiology
,
Lausanne University Hospital and University of Lausanne
,
2
Bioinformatics Core Facility
,
SIB Swiss Institute of Bioinformatics
Chapters
00:00
Introduction
00:07
Experimental Workflow
00:38
Total RNA Extraction
02:22
mRNA Isolation
04:38
mRNA Fragmentation
06:09
meRIP-Seq
10:21
Bisulfite Conversion
12:47
Results
13:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
RNA修饰在病毒感染中的作用才刚刚开始被探索,并可能突出新的病毒 - 宿主相互作用机制。在这项工作中,我们提供了一个管道来研究病毒感染背景下的
m6A
和
m5C
RNA修饰。
Tags
M6A
M5C
Epitranscriptome
Viral Infection
HIV
RNA Extraction
MRNA Isolation
Poly-A Selection
RNA-seq
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