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Cancer Research
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標的ナノ粒子の研究のための乳癌の同質性マウスモデルからの原発癌関連線維芽細胞の分離
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
標的ナノ粒子の研究のための乳癌の同質性マウスモデルからの原発癌関連線維芽細胞の分離
DOI:
10.3791/62504-v
•
08:02 min
•
May 14, 2021
•
Marta Truffi*
1
,
Leopoldo Sitia*
2
,
Marta Sevieri
,
Arianna Bonizzi
,
Maria Antonietta Rizzuto
,
Serena Mazzucchelli
,
Fabio Corsi
2
1
Istituti Clinici Scientifici Maugeri IRCCS
,
2
Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”
,
Università di Milano
,
3
Dipartimento di Biotecnologie e Bioscienze
,
Università di Milano-Bicocca
Chapters
00:04
Introduction
00:37
Tumor Dissociation into Single Cells
02:55
Extraction of Cancer-Associated Fibroblasts (CAFs) from Dissociated Tumor
05:20
Results-I: Isolation and Culture of Primary CAFs from a Syngeneic Murine Breast Cancer
06:40
Results-II: Use of Isolated CAFs to Evaluate Targeting Potential of Hfn-FAP Functionalized Nanodrugs
07:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
本論文は、三重陰性乳癌の同種マウスモデルからの原発性癌関連線維芽細胞の単離と培養と、腫瘍微小環境を標的とするように設計された新規ナノ粒子の前臨床試験への応用を目的とする。
Tags
Primary Cancer-associated Fibroblasts
Murine Breast Cancer
Tumor Microenvironment
In Vitro Studies
Tumor Stroma
Tumor Dissociation
Cell Isolation
Dead Cell Removal
Magnetic Separation
Cell Culture
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