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Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
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JoVE Journal Cancer Research
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

DOI:
10.3791/58101-v

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06:03 min

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January 19, 2019

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, , , , ,
1Department of Physiology, Center of Physiology and Pharmacology & Comprehensive Cancer Center (CCC),Medical University of Vienna, 2Ludwig Boltzmann Institute for Cancer Research (LBI-CR)

Chapters

  • 00:04Title
  • 00:57Orthotopic Transplantation via Intratracheal Delivery
  • 02:57Lung Preparation for Flow Cytometry
  • 04:54Results: Representative Mouse Lung Morphology Analyses Following Syngeneic, Intratracheal, Lung Adenocarcinoma Cell Transplantation
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a minimally invasive syngeneic orthotopic transplantation model of mouse lung adenocarcinoma cells as a time- and cost-reducing model to study non-small cell lung cancer.

Tags

Keywords: Orthotopic TransplantationSyngeneic Lung AdenocarcinomaPD-L1 ExpressionImmunocompetent MiceGene EditingTumor GrowthGene Expression ProfilesAutochthonous Lung Tumor ModelsMouse Lung Cancer ModelTracheal IntubationCell Suspension Delivery

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