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使用磁悬浮定量细胞密度和抗原特性
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Biology
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JoVE Journal
Biology
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
Please note that all translations are automatically generated.
Click here for the English version.
使用磁悬浮定量细胞密度和抗原特性
DOI:
10.3791/62550-v
•
05:25 min
•
May 17, 2021
•
Lauren Thompson
,
Brandy Pinckney
,
Shulin Lu
,
Mark Gregory
,
John Tigges
,
Ionita Ghiran
1
Nano Flow Core Facility
,
Beth Israel Deaconess Medical Center, Harvard Medical School
,
2
Department of Medicine, Division of Allergy and Inflammation
,
Beth Israel Deaconess Medical Center
Chapters
00:04
Introduction
00:43
Collection and Preparation of Blood for Positive Rhesus Factor Detection
02:43
Cell Analysis Using the Magnetic Levitation Device
03:21
Results: Analysis of the Separated Cells
04:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述了一种基于磁悬浮的方法,该方法可以通过量化具有固定密度的捕获微球悬浮高度的变化来特异性检测抗原的存在,无论是可溶的还是膜结合的。
Tags
Magnetic Levitation
Cell Density
Antigenic Properties
Minimally Invasive
Rhesus Factor
Fluorescent Dye
DPBS
HBSS
Gadolinium
IgG Control Beads
Anti-RhD Coated Beads
Capillary Tube
Cell Separation
Cell Detection
Anemia
Sepsis
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