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Cellular Redox Profiling Using High-content Microscopy
 

Cellular Redox Profiling Using High-content Microscopy

Article doi: 10.3791/55449
May 14th, 2017

Summary May 14th, 2017

This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).

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