Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Article DOI: 10.3791/55449
May 14th, 2017


Summary May 14th, 2017

This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).


Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter