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Cellular Redox Profiling Using High-content Microscopy
Cellular Redox Profiling Using High-content Microscopy
JoVE Journal
Biology
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JoVE Journal Biology
Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

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11:37 min

May 14, 2017

DOI:

11:37 min
May 14, 2017

10896 Views
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Summary

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This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).

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