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JoVE Journal Chemistry
Identifiering av antibakteriella immunitetsproteiner i Escherichia coli med MALDI-TOF-TOF-MS/MS och top-down proteomisk analys
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Identifiering av antibakteriella immunitetsproteiner i Escherichia coli med MALDI-TOF-TOF-MS/MS och top-down proteomisk analys

Article DOI: 10.3791/62577-v • 09:26 min • May 23rd, 2021
May 23rd, 2021 •
1, 1
1Produce Safety & Microbiology, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture

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Här presenterar vi ett protokoll för snabb identifiering av proteiner som produceras av genomiskt sekvenserade patogena bakterier med MALDI-TOF-TOF tandem masspektrometri och uppifrån och ner proteomisk analys med programvara som utvecklats internt. Metastabila proteinjoner fragment på grund av aspartsyra effekten och denna specificitet utnyttjas för protein identifiering.

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Kemi nummer 171
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