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Journal / Genetics / Next Generation Sequencing gebruiken om mutaties…
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JoVE Journal Genetics
Next Generation Sequencing gebruiken om mutaties te identificeren die verband houden met reparatie van een cas9-geïnduceerde dubbelstrengsbreuk in de buurt van de CD4-promotor
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Next Generation Sequencing gebruiken om mutaties te identificeren die verband houden met reparatie van een cas9-geïnduceerde dubbelstrengsbreuk in de buurt van de CD4-promotor

Article DOI: 10.3791/62583-v • 06:59 min • March 31st, 2022
March 31st, 2022 •
*1, *2, 1, 1, 2,3
1Division of Biology, Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 3Department of Diagnostic Medicine/Pathobiology, Kansas State University
* These authors contributed equally

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Hier gepresenteerd is sgRNA / CAS9 endonuclease en next-generation sequencing protocol dat kan worden gebruikt om de mutaties te identificeren die geassocieerd zijn met dubbelstrengs breukherstel in de buurt van de CD4-promotor.

Tags

Genetica DNA damage response DSB DSB repair CAS9 induced DSB Next-generation sequencing genomics
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