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使用 D11 scFv-碱性磷酸酶融合蛋白和免疫荧光直接检测组织中的异左旋蛋白
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
Journal (Biology)
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior
Journal (Biology)
A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior
 
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使用 D11 scFv-碱性磷酸酶融合蛋白和免疫荧光直接检测组织中的异左旋蛋白

Article DOI: 10.3791/62603-v • 06:33 min • July 5th, 2021
July 5th, 2021 •
*1, *2, 3, 4,6, 4, 5, 3, 4,6
1Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Department of Biochemistry, Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, 5Division of Hematology and Oncology, Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics, Vanderbilt University
* These authors contributed equally

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本文提供了使用碱性磷酸酶偶联的 ScFv D11 抗体通过免疫荧光测量组织中异左旋前列腺素的详细方法。小鼠和人类的高血压模型用于解释与组织样本中异左旋蛋白测量相关的分步程序和基本原理。

Tags

生物学，第 173 期，异左旋蛋白、免疫荧光、碱性磷酸酶偶联 ScFv D11 抗体和高血压
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