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使用单分子DNA拉伸含量直接观察DNA复制的酶
JoVE Journal
Biology
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JoVE Journal
Biology
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用单分子DNA拉伸含量直接观察DNA复制的酶
DOI:
10.3791/1689-v
•
17:03 min
•
March 23, 2010
•
Arkadiusz W. Kulczyk
,
Nathan A. Tanner
,
Joseph J. Loparo
,
Charles C. Richardson
,
Antoine M. van Oijen
1
Harvard Medical School
Chapters
00:00
Title
00:51
Introduction
01:18
Preparation of the DNA Replication Template
03:41
Bead Preparation
05:28
Preparing Functionalized Coverslips
08:31
Flow Chamber Assembly
11:34
Single-Molecule Replication Experiment
15:41
Single-Molecular Replication Data Analysis and Representative Results
16:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个观察单个DNA分子介导的蛋白质的噬菌体复制系统的实时复制的方法。
Tags
Enzymes
Replicating DNA
Single-molecule DNA Stretching Assay
Bacteriophage Replication System
Biotin
Digoxigenin
Functionalized Glass Coverslip
Small Bead
DNA-bead Tethers
Flow Cell
Laminar Flow
Drag Force
Stretched DNA
Coverslip Surface
Flow Rate
Length Measurement
Single-stranded DNA
Double-stranded DNA
Replication Proteins
Fork
Unwinding
Polymerization
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