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Biology

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Directe detectie van isolevuglandinen in weefsels met behulp van een D11 scFv-alkalisch fosfatasefusie-eiwit en immunofluorescentie
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Directe detectie van isolevuglandinen in weefsels met behulp van een D11 scFv-alkalisch fosfatasefusie-eiwit en immunofluorescentie

Article DOI: 10.3791/62603-v • 06:33 min • July 5th, 2021
July 5th, 2021 •
*1, *2, 3, 4,6, 4, 5, 3, 4,6
1Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Department of Biochemistry, Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, 5Division of Hematology and Oncology, Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics, Vanderbilt University
* These authors contributed equally

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Dit artikel biedt een gedetailleerde methodologie voor de meting van isolevuglandinen in weefsels door immunofluorescentie met behulp van alkalische fosfatase-geconjugeerde ScFv D11-antilichamen. Hypertensiemodellen bij zowel muizen als mensen worden gebruikt om de stapsgewijze procedures en fundamentele principes uit te leggen die verband houden met isolevuglandinemeting in weefselmonsters.

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Biologie isolevuglandinen immunofluorescentie alkalische fosfatase geconjugeerd ScFv D11-antilichaam en hypertensie
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