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JoVE Journal Biology
Direkte påvisning af isolevuglandiner i væv under anvendelse af et D11 scFv-alkalisk fosfatasefusionsprotein og immunofluorescens
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Direkte påvisning af isolevuglandiner i væv under anvendelse af et D11 scFv-alkalisk fosfatasefusionsprotein og immunofluorescens

Article DOI: 10.3791/62603-v • 06:33 min • July 5th, 2021
July 5th, 2021 •
*1, *2, 3, 4,6, 4, 5, 3, 4,6
1Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Department of Biochemistry, Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, 5Division of Hematology and Oncology, Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics, Vanderbilt University
* These authors contributed equally

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Denne artikel indeholder en detaljeret metode til måling af isolevuglandiner i væv ved immunofluorescens under anvendelse af alkalisk phosphatase-konjugeret ScFv D11-antistof. Hypertensionsmodeller hos både mus og mennesker bruges til at forklare de trinvise procedurer og grundlæggende principper forbundet med isolevuglandinmåling i vævsprøver.

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Biologi udgave 173 isolevuglandiner immunofluorescens alkalisk fosfatasekonjugeret ScFv D11-antistof og hypertension
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