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Journal / Bioengineering / Fibroblast avledet menneskekonstruert bindevev…
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JoVE Journal Bioengineering
Fibroblast avledet menneskekonstruert bindevev for screeningapplikasjoner
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Fibroblast avledet menneskekonstruert bindevev for screeningapplikasjoner

Article DOI: 10.3791/62700-v • 09:50 min • August 20th, 2021
August 20th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2,3,4,5, 1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)

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Presentert her er en protokoll for å generere konstruert bindevev for en parallell kultur på 48 vev i en multi-brønn plate med doble poler, egnet for mekanistiske studier, sykdomsmodellering og screening applikasjoner. Protokollen er kompatibel med fibroblaster fra forskjellige organer og arter og eksemplifiseres her med humane primære hjertefibroblaster.

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Bioingeniør Utgave 174 vevsteknikk hjertefibroblast kollagenmatrise vevsstivhet screening
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