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JoVE Journal Bioengineering
Fibroblast afledte humane manipuleret bindevæv til screeningsapplikationer
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Fibroblast afledte humane manipuleret bindevæv til screeningsapplikationer

Article DOI: 10.3791/62700-v • 09:50 min • August 20th, 2021
August 20th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2,3,4,5, 1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)

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Præsenteret her er en protokol til at generere manipuleret bindevæv til en parallel kultur på 48 væv i en multi-brønd plade med dobbelt poler, egnet til mekanistiske undersøgelser, sygdom modellering, og screening applikationer. Protokollen er kompatibel med fibroblaster fra forskellige organer og arter og er eksemplificeret her med humane primære hjertefibroblaster.

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Bioengineering vævsteknik hjertefibroblast kollagenmatrix vævsstivhed screening
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