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1The Brady Urological Institute,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences,Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital,Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter,Institute for Basic Science (IBS)
Chapters
- 00:04Introduction
- 01:15Extracellular Vesicle (EV) Isolation and On-Chip Immuno-Fluorescent Labeling
- 02:54Incubation of the Cells with Fluorescently Labeled EVs for the EV-Uptake Assay
- 03:45Confocal Microscopy and Image Processing
- 06:28Results: EV Uptake Assay Using 3D Fluorescence Imaging via Confocal Microscopy
- 07:31Conclusion
Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.
