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Bioengineering
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リセートベースの無細胞システムにおける代謝物プロファイリングのためのリキッドクロマトグラフィー結合と屈折率または質量分析法検出
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Please note that all translations are automatically generated.
Click here for the English version.
リセートベースの無細胞システムにおける代謝物プロファイリングのためのリキッドクロマトグラフィー結合と屈折率または質量分析法検出
DOI:
10.3791/62852-v
•
14:42 min
•
September 23, 2021
•
Jaime Lorenzo N. Dinglasan
3
,
David T. Reeves
3
,
Robert L. Hettich
,
Mitchel J. Doktycz
1
Graduate School of Genome Science & Technology
,
University of Tennessee Knoxville
,
2
Bredesen Center for Interdisciplinary Research
,
University of Tennessee Knoxville
,
3
Biosciences Division
,
Oak Ridge National Laboratory
Chapters
00:04
Introduction
01:08
Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification
03:18
Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition
04:54
Extracting and Analyzing Data Post-Run
07:20
Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification
09:20
Setting Up a Run Sequence and Starting the LC-MS/MS Run
10:16
Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data
11:45
Results: Metabolite Profiling in Lysate-based Cell-free Systems
13:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
プロトコルは、複雑な溶解物ベースの無細胞系における代謝反応を研究するための屈折率または質量分析検出に結合された高速液体クロマトグラフィー法を記述する。
Tags
Liquid Chromatography
Refractive Index Detection
Mass Spectrometry
Metabolite Profiling
Cell-free Systems
Carbon Metabolism
Central Metabolites
Lysate-based
HPLC
Sample Preparation
Acidification
Filtration
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