Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Cellefri proteinsyntese fra…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Cellefri proteinsyntese fra exonuklease-mangelfulde cellulære ekstrakter ved hjælp af lineære DNA-skabeloner
Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
Journal (Bioengineering)
Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
Journal (Bioengineering)
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
 
Click here for the English version

Cellefri proteinsyntese fra exonuklease-mangelfulde cellulære ekstrakter ved hjælp af lineære DNA-skabeloner

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenteres en protokol til fremstilling og bufferkalibrering af celleekstrakter fra exonuklease V knockout-stammer af Escherichia coli BL21 Rosetta2 (ΔrecBCD og ΔrecB). Dette er en hurtig, nem og direkte tilgang til ekspression i cellefrie proteinsyntesesystemer ved hjælp af lineære DNA-skabeloner.

Tags

Bioengineering udgave 186
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter