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通过选择性核糖体分析对共翻译相互作用网络进行全局鉴定

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Biology

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JoVE Journal Biology

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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通过选择性核糖体分析对共翻译相互作用网络进行全局鉴定

DOI:

10.3791/62878-v

•

06:58 min

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October 07, 2021

•

Johannes Venezian, Hila Zilberman, Ayala Shiber

¹Faculty of Biology,Technion – Israel Institute of Technology

Chapters

00:04Introduction
00:49Cell Collection and Lysis
01:29Processing the Total RNA Sample
02:24Processing the Immunopurification Sample
04:34Results: Analysis of Ribosome-Protected Footprint Isolation, RNA Extraction, cDNA Library Creation, and Co-Translational Interactions
06:19Conclusion

Summary

Automatic Translation

共转化相互作用在新生链修饰，靶向，折叠和组装途径中起着至关重要的作用。在这里，我们描述了选择性核糖体分析，这是一种体内直接分析真核生物酿酒酵母模型中这些相互作用的方法。

Tags

Keywords: Co-translational Interactions Selective Ribosome Profiling In Vivo Analysis Saccaromyces Cerevisiae Cryogenic Grinding RNase I Digestion Sucrose Cushion Affinity Purification Protein-ribosome Interactions Global Profiling

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