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Quantifying Levels of Dopaminergic Neuron Morphological Alteration and Degeneration in Caenorhabditis elegans
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Neuroscience
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JoVE Journal Neuroscience
Quantifying Levels of Dopaminergic Neuron Morphological Alteration and Degeneration in Caenorhabditis elegans

Quantifying Levels of Dopaminergic Neuron Morphological Alteration and Degeneration in Caenorhabditis elegans

DOI:
10.3791/62894-v

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06:17 min

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November 20, 2021

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, ,
1Nicholas School of the Environment,Duke University

Summary

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In this article, we showcase how to use a seven-point scoring system to consistently quantify changes to dopaminergic neuron dendrite morphology in C. elegans. This system is intended for analyses of dopaminergic neurodegeneration assays utilizing genetic, chemical, and age-based models of neurodegenerative disorders.

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Keywords: QuantifyingDopaminergicNeuronMorphologicalAlterationDegenerationCaenorhabditis ElegansScoring SystemDendrite MorphologyNeurodegenerationImagingFluorescenceZ-stackDendrite ScoringBlebsBreaksIrregularitiesRepeatability

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